| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Huber, Joshua D (15)
|
NITTAC |
2:32.48 |
2:33.60 |
1.12 |
| 2 |
Huber, Caleb J (15)
|
NITTAC |
2:37.20 |
2:41.59 |
4.39 |
| 3 |
Rozler, Rennee A (16)
|
NIHAMB |
2:55.15 |
2:46.93 |
-8.22 |
| 4 |
Diaz, Lexie L (17)
|
NIGISC |
2:30.69 |
2:48.56 |
17.87 |
| 5 |
Zawodzinski, Zoe E (13)
|
NIHAMB |
|
2:50.41 |
- |
| 6 |
Stockschlaeder, Amelia R (17)
|
NIHAMB |
|
2:50.48 |
- |
| 7 |
Clemens, Megan E (16)
|
NITTAC |
3:00.21 |
2:59.51 |
-0.70 |
| 8 |
Sauer, Emma L (13)
|
NIHAMB |
|
3:10.81 |
- |
| 9 |
Sellan, Sophia K (11)
|
NITTAC |
4:10.21 |
4:06.31 |
-3.90 |
|
Greier, Devon P (16)
|
NITTAC |
3:06.41 |
NS |
- |
|
Yandricha, Elizabeth R (10)
|
NITTAC |
3:40.21 |
NS |
- |